Hooke's highly experienced histology team offers a comprehensive set of histology, IHC, and pathology services.
We accept fixed and frozen samples, embedded tissue blocks, unstained or stained slides, or digital images for analysis.
Available outputs include tissue blocks, mounted slides, and high-resolution digital images of your slides (Hamamatsu NanoZoomer). We can also perform statistical analysis and generate reports.
We routinely process and stain both formalin-fixed and cryopreserved tissue.
Hooke can decalcify fixed tissue, prepare optimal cutting temperature compound (OCT) blocks or formalin-fixed paraffin embedded (FFPE) blocks, and make slides.
Hooke's validated stains include:
Other stains can be obtained on request.
Hooke has validated the following IHC marker antibodies:
We can also work with you to validate additional markers.
EAE was induced in C57BL/6 mice by immunization with MOG35-55 + PTX; analysis was on Day 28 after immunization. Treatments FTY720 (positive control, left) and vehicle (negative control, right) were administered prophylactically (from EAE induction), p.o. QD. Tissues were collected, fixed, processed and stained for myelin basic protein (MBP; brown). Scale bars 250 µm.
Healthy (CD4 transferred mice; left) and colitis-induced (CD45RBhigh transferred mice; right) mice were sacrificed at 42 days post-colitis induction. Colons were cleaned, fixed, and immunohistochemically stained for anti-phospho-STAT3 (aP-STAT3, brown). Scale bar 2.5 mm for full size image, 50 µm for insets.
We offer pathological scoring of rodent spine, colon, kidney, spleen (including follicle counts), paw, and ear tissue (including dermal thickness measurement) for inflammatory and autoimmune disease models, including:
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