By active immunization
Emulsion supplied in pre-filled syringes, ready to use.
EAE will develop in 90 to 100% of rats within 10 to 14 days of immunization. Maximum score for individual rats will typically be 3.0 to 3.5.
Each lot is tested and individually adjusted to ensure consistent disease induction.
Properly prepared emulsions are critical for reliable induction of many autoimmune disease models. Our emulsions are carefully made and pre-filled into syringes, ready to use, to reduce time needed to set up experiments.
Lot-to-lot reagent variations can cause dramatic changes in severity of induced autoimmune disease. The consistent potency of pre-characterized Hooke Kits™ eliminates time-consuming testing.
Pre-filled syringes are prepared under aseptic conditions and delivered in sterilized plastic bags for easy disinfection before introduction into your rodent facility.
Experimental autoimmune encephalomyelitis (EAE) is the model most commonly used to study efficacy of potential drugs for treatment of multiple sclerosis (MS).
The EAE model in Lewis rats has consistent disease onset and severity, as well as a short clinical course, making it a popular compound screen for potential efficacy in MS.
EAE in Lewis rats is characterized by paralysis and CNS inflammation. Demyelination is not typically observed. EAE is initiated by myelin-specific CD4+ T cells, with glia cells (microglia and astrocytes), B cells, macrophages, NK cells, dendritic cells, and mast cells all likely playing roles in disease development.
Most investigators immunize Lewis rats with gpMBP69-88 to induce EAE (cat. no. EK-3110, without pertussis toxin). In this model rats fully recover by Day 20 after immunization.
It is possible to induce more severe EAE by administering pertussis toxin (PTX) to rats one day after immunization. If PTX is administered in addition to the antigen, 30% to 60% of rats will experience a relapse within 28 days from immunization. This provides an opportunity for study of mechanism of EAE development and relapses.
For more information on model selection, please see Learning Center - What are the advantages and disadvantages of the different EAE models and antigens?.
Hooke kits™ can be customized for a small additional charge. Contact us at with your requirements.
Each kit provides sufficient reagents for 10 rats.
Antigen is myelin basic protein 69-88, guinea pig (gpMBP69-88), sequence YGSLPQKSQRSQDENPVVHF.
| Qty | Description |
|---|---|
| 3 | Syringes pre-filled with 0.7 mL gpMBP69-88/CFA emulsion ~ 0.5 mg gpMBP69-88/mL emulsion ~ 2-5 mg killed mycobacterium tuberculosis H37Ra/mL emulsion (all concentrations adjusted by lot for consistent EAE induction) |
| 1 | Data sheet: Recommended experimental protocol, typical results |
[1] McFarlin DE, Blank SE, Kibler RF, J. Immunol. 1 13:712 (1974)
[2] Kardys E and Hashim GA, J Immunol 127:862 (1981)
[3] Mannie MD, Paterson PY, U'Prichard DC, Flouret G., Proc Natl Acad Sci USA 82:5515 (1985)
[4] Hashim GA, Day ED, Fredane L, Intintola P, Carvalho E, J Neurosci Res. 16(3):467-78 (1986)
CK-3110
Hooke Control Kit™ for EK-3110 (full size)
CK-8110
Hooke Control Kit™ for EK-3110 (half size)