Tissue culture tested

In tissue culture media, ready to use

Supplied in vials

For analysis of T cell functions in vitro

Consistently induces in vitro recall responses of T cells isolated from MBP_1-17/CFA-immunized mice

For adoptive transfer EAE in B10.PL, PL/J, or anti-MBP TCR transgenic mice

Induces potent encephalitogenic T cells in vitro

MBP_1-17 in TC media is used in vitro to generate encephalitogenic T cells or to evaluate cellular immune responses.

Encephalitogenic cells are generated by culturing splenocytes from immunized mice for 72 hours in the presence of 20 µg/mL of antigen. To generate encephalitogenic cells, IL-23 or IL-12 and anti-IFNγ antibodies may be added to the culture.

Protocol

Cytokine Production Induced by T Cell Recall Response In Vitro

Detailed contents

Myelin basic protein 1-17 (MBP_1-17)
2 mg in 1 mL of tissue culture media (10% fetal bovine serum in RPMI 1640)
Mouse sequence ASQKRPSQRSKYLATAS
Supplied in vials, ready to use

References

Perrin PJ et al, J Immunol 163;1704 (1999)
Sabelko-Downes KA et al, J Exp Med 189:1195 (1999)
Lafaille JJ et al, J Exp Med 186:307 (1997)

Safety Data Sheet (SDS)

(PDF)

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