Tissue culture tested

In tissue culture media, ready to use

Supplied in vials

For analysis of T cell functions in vitro

Consistently induces in vitro recall responses of T cells isolated from [Ser¹⁴⁰]-PLP_139-151/CFA-immunized mice

For adoptive transfer EAE in SJL mice

Induces potent encephalitogenic T cells in vitro

[Ser¹⁴⁰]-PLP_139-151 in TC media is used in vitro to generate encephalitogenic T cells or to evaluate cellular immune responses.

Encephalitogenic cells are generated by culturing splenocytes and lymph node cells from immunized mice for 72 hours in the presence of 20 µg/mL of antigen.

Protocols

Adoptive Transfer EAE in SJL Mice
Cytokine Production Induced by T Cell Recall Response In Vitro

Detailed contents

[Ser¹⁴⁰]-PLP_139-151 (serine substituted myelin proteolipid protein 139-151)
2 mg in 1 mL of tissue culture media (10% fetal bovine serum in RPMI 1640)
Sequence HSLGKWLGHPDKF
Supplied in vials, ready to use

References

Leonard JP et al., Crit Rev Immunol 17:545 (1997)
McRae BL et al., J Neuroimmunol 38:229 (1992)
Tuohy VK et al., J Immunol 142:1523 (1989)
Nicholson LB et al., Proc Natl Acad Sci USA 95:264 (1998)

Safety Data Sheet (SDS)

(PDF)

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